Production of bacterial cellulose nanocrystals via enzymatic hydrolysis and evaluation of their coating on alginate particles formed by ionotropic gelation
Victória Soares Soeiro, Louise Lacalendola Tundisi, Letícia C.L. Novaes, Priscila Gava Mazzola, Norberto Aranha, Denise Grotto, José Martins de Oliveira, Daniel Komatsu, Miguel Gama, Marco V. Chaud, Ângela Faustino Jozala
Abstract
This study aimed to obtain the bacterial cellulose nanocrystals (CNC) by enzymatic hydrolysis and verify the CNC application as coating material in alginate particles. Therefore, the production of CNC was carried out through two enzymatic hydrolysis methods involving a time period of 48 and 72 h. 0.35 mg of dry mass of cellulose was produced approximately 1.6 × 1011 CNC/mL. The CNC obtained by enzymatic hydrolysis at 72 h (Method II) was applied to cover the alginate particles, obtained by ionotropic gelation. The CNC Zeta potential value was about +15 mV and for alginate particles -26.46 ± 1.48 mV. These results confirmed the application of CNC as coating material for alginate particles. It brings an incremental contribution to the knowledge advancement in the pharmaceutical and food area, allowing the engineering of systems to use a mixed composition of nanobiomaterials to modify the release pattern of drugs, macromolecule, nutrients, stabilizers and target specific drug release.