Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts
V. A. Sidorov, Dafu Wang, É. Nagy, Charles Armstrong, Steven R. H. Beach, Yuanji Zhang, Jeanna Groat, Sam Yang, Peizhen Yang, Larry A. Gilbertson
Topics & Concepts
ProtoplastCanolaGenome editingBiologyBrassicaTransformation (genetics)Cas9DNACallusGenomeGeneticsGeneBotanyCRISPR and Genetic EngineeringChromosomal and Genetic VariationsRNA and protein synthesis mechanisms