Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa
Adam Kawałek, Krzysztof Głąbski, Aneta Agnieszka Bartosik, Dominika Woźniak, Magdalena Kusiak, Jan Gawor, Karolina Żuchniewicz, Grażyna Jagura‐Burdzy
Abstract
system is involved in the precise separation of newly replicated bacterial chromosomes. In this work, we identified and characterized proteins interacting with partitioning protein ParB. We mapped the domain of interactions with its cognate ParA partner and showed that ParB-ParA interactions are crucial for the chromosome segregation and for proper SMC action on DNA. We also demonstrated ParB interactions with other DNA binding proteins, metabolic enzymes, and NTPases displaying polar localization in the cells. Overall, this study uncovers novel players cooperating with the chromosome partition system in P. aeruginosa, supporting its important regulatory role in the bacterial cell cycle.