Rich dynamics and functional organization on topographically designed neuronal networks in vitro
Marc Montalà-Flaquer, Clara F. López-León, Daniel Tornero, Akke Mats Houben, Tanguy Fardet, Pascal Monceau, Samuel Bottani, Jordi Soriano
Abstract
using fluorescence calcium imaging. The networks were characterized by rich spatiotemporal activity patterns that comprised from small regions of the culture to its whole extent. Effective connectivity analysis revealed the emergence of spatially compact functional modules that were associated with both the underpinned topographical features and predominant spatiotemporal activity fronts. Our results show the capacity of spatial constraints to mold activity and functional organization, bringing new opportunities to comprehend the structure-function relationship in living neuronal circuits.
Topics & Concepts
Calcium imagingFunctional organizationNeurosciencePremovement neuronal activityBiological neural networkNerve netBurstingSpatial organizationFunctional connectivityBiologyNeuronal circuitsDynamics (music)Biological systemChemistryCalciumPhysicsEvolutionary biologyAcousticsOrganic chemistryNeuroscience and Neural Engineering3D Printing in Biomedical ResearchNeural dynamics and brain function