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Tailor-made β-glucosidase with increased activity at lower temperature without loss of stability and glucose tolerance

Florian Lenz, Paul Jannis Zurek, Martina Umlauf, Iasson E. P. Tozakidis, Joachim Jose

2020Green Chemistry26 citationsDOI

Abstract

We evolved the thermophilic β-glucosidase <italic>Cs</italic>BglA to 150% improvement of <italic>k</italic><sub>cat</sub>/<italic>K</italic><sub>M</sub> at 55 °C, exerting a specific activity of 504 U mg<sup>−1</sup> and a <italic>K</italic><sub>M</sub>-value of 37.1 mM, making it the bacterial β-glucosidase with highest activity known so far.

Topics & Concepts

ChemistryEnzyme Production and CharacterizationEnzyme Catalysis and ImmobilizationPancreatic function and diabetes
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