Diamine oxidase-modified screen-printed electrode for the redox-mediated determination of histamine
Ricarda Torre, Estefanía Costa‐Rama, Henri P.A. Nouws, Cristina Delerue‐Matos
Abstract
Abstract Histamine is an important biogenic amine because of its role in immune responses and the regulation of physiological functions. It is also used as a food freshness indicator, so its maximum concentration in fish is legally regulated. Although several robust and sensitive methods for histamine detection are already available, it continues to be a challenge to develop simple and portable devices that allow rapid histamine screening at any point of the fish production chain. Thus, in this work, a simple, miniaturized and low-cost sensor for histamine analysis was developed. The construction of the sensor only takes 30 min and consists of the immobilization of the enzyme diamine oxidase on the surface of a screen-printed carbon electrode by cross-linking. The quantification of histamine was achieved by chronoamperometry (+ 0.2V, 120 s) using hexacyanoferrate (III) as a redox mediator. This selective sensor provided a low limit of detection (0.97 mg L −1 ) and accurate and precise results and was successfully applied to the analysis of spiked tuna and mackerel extracts, obtaining recovery values of 99–100%. Moreover, the sensor shows good stability, maintaining 87.7% of its initial signal after 35 days.