CK2 Phosphorylation of Human Papillomavirus 16 E2 on Serine 23 Promotes Interaction with TopBP1 and Is Critical for E2 Interaction with Mitotic Chromatin and the Viral Life Cycle
Apurva T. Prabhakar, Claire D. James, Dipon Das, Raymonde Otoa, M.W. Day, John W. Burgner, Christian T. Fontan, Xu Wang, Sarah H. Glass, Andreas Wieland, Mary Donaldson, Molly L. Bristol, Renfeng Li, Anthony W. Oliver, Laurence H. Pearl, Brian O. Smith, Iain M. Morgan
Abstract
Human papillomaviruses are causative agents in around 5% of all cancers, with no specific antiviral therapeutics available for treating infections or resultant cancers. In this report, we demonstrate that phosphorylation of HPV16 E2 by CK2 promotes formation of a complex with the cellular protein TopBP1 in vitro and in vivo .
Topics & Concepts
ChromatinCell biologyMitosisPhosphorylationSerineHuman papillomavirusViral life cycleVirologyBiologyChemistryDNAMedicineGeneticsViral replicationVirusInternal medicineCancer-related Molecular PathwaysCervical Cancer and HPV ResearchCancer Diagnosis and Treatment