Heat stress induced IPEC‐J2 cells barrier dysfunction through endoplasmic reticulum stress mediated apoptosis by p‐eif2α/CHOP pathway
Yanjun Cui, Leyi Chen, Xu Zhou, Zhining Tang, Chong Wang, Haifeng Wang
Abstract
Abstract Heat stress (HS) induced by high ambient temperatures compromises intestinal epithelial cell. However, the underlying mechanisms by which HS causes intestinal barrier dysfunction remain unclear. In this study, we established an in vitro acute‐HS‐induced intestinal damage using porcine small intestinal epithelial cell (IPEC‐J2) that exposed to the high temperatures (43°C) for 2 h. The cell proliferation, apoptosis, tight junction (TJ) barrier integrity and transcriptomic profiles were measured. The results showed that HS decreased cell viability while increased proapoptotic signaling evidenced by Bax/bcl2 ratio, cytochrome C release to cytosol and active‐caspase 3 increases ( p < 0.01). HS led to decreased transepithelial electrical resistance, increased cell permeability, and downregulated TJ proteins including ZO1, occludin, and claudin 3 ( p < 0.05). Transcriptome sequencing and KEGG pathway analysis revealed HS‐induced cell cycle arrest and activation of endoplasmic reticulum stress (ERS) response mediated by a critical transcript eif2α and proapoptotic molecule DDIT3 (known as CHOP). Furthermore, inhibition of ERS by 4‐phenylbutyrate (4‐PBA) administration and knockdown of eif2α and CHOP significantly attenuated IPEC‐J2 cells apoptosis ( p < 0.05). Transmission electron microscopy analysis suggested that 4‐PBA inhibited HS‐induced increase in ER lumen diameter, indicating ultrastructural sign of ERS. In addition, HS‐induced impairment of TJs was significantly attenuated by 4‐PBA ( p < 0.05). Collectively, HS induces ERS and activates the p‐eif2α/CHOP signaling pathway to impair epithelial barrier integrity through triggering the intestinal epithelial cell apoptosis.