COVID-19 and Diagnostic Testing for SARS-CoV-2 by RT-qPCR—Facts and Fallacies
Stephen A. Bustin, Reinhold Mueller, Gregory L. Shipley, Tania Nolan
Abstract
Although molecular testing, and RT-qPCR in particular, has been an indispensable component in the scientific armoury targeting SARS-CoV-2, there are numerous falsehoods, misconceptions, assumptions and exaggerated expectations with regards to capability, performance and usefulness of the technology. It is essential that the true strengths and limitations, although publicised for at least twenty years, are restated in the context of the current COVID-19 epidemic. The main objective of this commentary is to address and help stop the unfounded and debilitating speculation surrounding its use.
Topics & Concepts
Coronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Speculation2019-20 coronavirus outbreakContext (archaeology)Diagnostic testPandemicMedicineIntensive care medicineVirologyData scienceBiologyComputer sciencePathologyDiseaseEconomicsOutbreakInfectious disease (medical specialty)Veterinary medicineMacroeconomicsPaleontologySARS-CoV-2 detection and testingBiosensors and Analytical DetectionMolecular Biology Techniques and Applications