Metallic micro-ring device for highly efficient large cargo delivery in mammalian cells using infrared light pulses
Ashwini Shinde, Pallavi Shinde, Srabani Kar, Kavitha Illath, Souvik Dey, Nitish R. Mahapatra, Moeto Nagai, Tuhin Subhra Santra
Abstract
laser fluence for 250 pulses in SiHa cells. Moreover, the intracellular delivery of β-galactosidase enzyme was confirmed with confocal microscopy and flow cytometry analysis resulting in ∼83% co-staining of β-galactosidase enzyme and calcein AM. Based on these efficient deliveries of diverse types of biomolecules in different cell types, the device has the potential for cellular diagnostic and therapeutic applications.
Topics & Concepts
InfraredMetalMaterials scienceOptoelectronicsRing (chemistry)NanotechnologyChemistryOpticsPhysicsMetallurgyOrganic chemistryMicrofluidic and Bio-sensing TechnologiesSpectroscopy Techniques in Biomedical and Chemical Research3D Printing in Biomedical Research