Noc Corrals Migration of FtsZ Protofilaments during Cytokinesis in Bacillus subtilis
Yuanchen Yu, Jinsheng Zhou, Frederico J. Gueiros‐Filho, Daniel B. Kearns, Stephen C. Jacobson
Abstract
, nucleoid occlusion has been reported to be mediated, at least in part, by the DNA-membrane bridging protein, Noc. Using time-lapse fluorescence microscopy of cells growing in microchannels, we show that Noc neither protects the chromosome from proximal Z-ring formation nor determines the future site of cell division. Rather, Noc plays a corralling role by preventing protofilaments from leaving a Z-ring undergoing cytokinesis and traveling over the nucleoid.
Topics & Concepts
FtsZNucleoidBacillus subtilisCytokinesisChromosome segregationCell divisionCell biologyChemistryBacterial cell structureDNACircular bacterial chromosomeCellBacteriaBiophysicsBiologyDNA replicationChromosomeEscherichia coliGeneticsBiochemistryGeneBacterial Genetics and BiotechnologyBacteriophages and microbial interactionsRNA and protein synthesis mechanisms