A programmable and automated optical electrowetting-on-dielectric (oEWOD) driven platform for massively parallel and sequential processing of single cell assay operations
Lawrence G. Welch, Jasper Estranero, Panagiotis Tourlomousis, Robert C. R. Wootton, Valentin Radu, C. Gónzalez-Fernández, Tim J. Puchtler, Claire M. Murzeau, Nele M. G. Dieckmann, Aya Shibahara, Brooke W. Longbottom, Clare Bryant, Emma L. Talbot
Abstract
single cell droplets) are retained for analysis, thereby overcoming the Poisson probability distribution. Droplets are stored in an array on a temperature-controlled chip and the history of individual droplets is logged from the point of filter until completion of the workflow. On chip, droplets are subject to an automated and flexible suite of operations including the merging of sample droplets and the fluorescent acquisition of assay readouts to enable complex sequential assay workflows. To demonstrate the broad utility of the platform, we present examples of single-cell functional workflows for various applications such as antibody discovery, infectious disease, and cell and gene therapy.