Nanozyme Sensor Array Based on Porphyrin-Modified CoMoO<sub>4</sub> to Detect and Distinguish Biologically Important Thiols
Pingping Hao, Yaru Liu, Liming Wang, Xixi Zhu, Qingyun Liu
Abstract
In order to establish a rapid, accurate, and simple method for monitoring and analyzing the biological physiological state, we constructed a colorimetric sensor array based on the peroxidase-like activity of nanozymes (CoMoO 4 and Por-CoMoO 4 ) for the determination of five kinds of biothiols. The peroxidase-like activity of CoMoO 4 has been greatly enhanced by the introduction of porphyrin (Por), due to the synergistic interaction between porphyrin and CoMoO 4 . Experimental data of the radical scavenger and fluorescence probe verify the generation of a superoxide radical (•O 2 – ) during the catalytic reaction. Because different biothiols have different inhibiting effects on the catalytic activity of two nanozymes, each biothiol has a unique fingerprint with the aid of a chromogenic substrate (TMB). Through linear discriminant analysis, captopril (CAP), cysteine (Cys), homocysteine (Hcy), dithiothreitol (DTT), and glutathione (GSH) can be well identified in a certain range of concentrations. Different concentrations of biothiols and different proportions of mixed biothiols can be identified. It is expected that this study will provide a simple and rapid method for the identification of structural analogues, contributing to the monitoring of human life and health.