One-Step Synthesis of Peptide–Gold Nanoclusters with Tunable Fluorescence and Enhanced Gene Delivery Efficiency
Zixuan Wang, Chuanmei Tang, Meimei Huang, Xi Rong, Hanqi Lin, Rongxin Su, Yuefei Wang, Wei Qi
Abstract
In this study, peptide-gold nanoclusters with tunable fluorescence were prepared by a simple "one-pot" method, which were used for gene localization and delivery in vivo to achieve efficient intracellular colocalization, uptake, and transfection. The efficiency of pDNA transfection was up to 70.6%, and there was no obvious cytotoxicity. This study proves that the simple-composition and bio-friendly peptide-gold nanoclusters are promising gene delivery carriers and can provide a powerful theoretical and experimental basis for the application of peptide-metal nanocomplexes in gene delivery and other biomedicine fields.
Topics & Concepts
NanoclustersGene deliveryColocalizationPeptideTransfectionFluorescenceChemistryNanotechnologyCytotoxicityIntracellularBiophysicsIn vivoCombinatorial chemistryMaterials scienceIn vitroGeneMolecular biologyBiochemistryBiologyBiotechnologyPhysicsQuantum mechanicsNanocluster Synthesis and ApplicationsAdvanced biosensing and bioanalysis techniquesAdvanced Nanomaterials in Catalysis