A general Fc engineering platform for the next generation of antibody therapeutics
Da Chen, Yingjie Zhao, Mingyu Li, Hang Shang, Na Li, Fan Li, Wei Wang, Yuan Wang, Ruina Jin, Shiyu Liu, Xun Li, Shan Gao, Yujie Tian, Ruonan Li, Huanhuan Li, Yongyan Zhang, Mingjuan Du, Youjia Cao, Yan Zhang, Xin Li, Yi Huang, Liaoyuan A. Hu, Fubin Li, Hongkai Zhang
Abstract
This approach increased the throughput of Fc variant screening from thousands to millions magnitude, enabled screening variants containing multiple mutations and could be integrated with glycoengineering technology, represents an ideal platform for Fc engineering. The initial efforts demonstrated the capability of the platform and the novel Fc variants could be substituted into nearly any antibody for the next generation of antibody therapeutics.
Topics & Concepts
AntibodyAntibody-dependent cell-mediated cytotoxicityFragment crystallizable regionHigh-throughput screeningPhage displayMonoclonal antibodyProtein engineeringAffinity maturationChemistryComputational biologyIn silicoMolecular biologyCell biologyBiologyImmunologyBiochemistryGeneEnzymeMonoclonal and Polyclonal Antibodies ResearchTransgenic Plants and ApplicationsProtein purification and stability