Plasmid encoding microRNA-200c ameliorates periodontitis and systemic inflammation in obese mice
Tadkamol Krongbaramee, Min Zhu, Qingwen Qian, Zeyuan Zhang, Steven Eliason, Yi Shu, Fang Qian, Adil Akkouch, Dan Su, Brad A. Amendt, Ling Yang, Liu Hong
Abstract
. Injection of naked plasmid DNA encoding miR-200c into the gingiva effectively rescued miR-200c downregulation, prevented periodontal and systemic inflammation, and alleviated the impaired glucose metabolism in obese mice with LPS-induced periodontitis. Increased circulating exosomal miR-200c and its function on suppressing proinflammatory cytokines and adipogenesis explained the mechanism(s) of gingival application of miR-200c in attenuating systemic inflammation in PiOSs. These results demonstrated that miR-200c reduced by Pg-LPS and IL-6 in periodontitis and obesity might lead to the pathogenesis of PiOSs, and upregulation of miR-200c in the gingiva presents a therapeutic approach for PiOSs.