Antiviral Evaluation of 1,4-disubstituted-1,2,3-triazole Derivatives Against Chikungunya Virus
Vitor Won‐Held Rabelo, Verônica Diniz da Silva, Maria Leonisa Sanchez Nuñez, Leonardo dos Santos Corrêa Amorim, Camilla D. Buarque, Richard Kühn, Paula Alvarez Abreu, Izabel Christina Nunes de Palmer Paixão
Abstract
Aim: ) against Chikungunya virus (CHIKV) replication. Materials & methods: Cytotoxicity was analyzed using colorimetric assays and the antiviral potential was evaluated using plaque assays and computational tools. Results: Compound 2 showed antiviral activity against CHIKV 181-25 in BHK-21 and Vero cells. Also, this compound presented a higher activity against CHIKV BRA/RJ/18 in Vero cells, like compound 1. Compound 2 exhibited virucidal activity and inhibited virus entry while compound 1 inhibited virus release. Molecular docking suggested that these derivatives inhibit nsP1 protein while compound 1 may also target capsid protein. Conclusion: Both compounds exhibit promising antiviral activity against CHIKV by blocking different steps of virus replication.