Staining the Ground Section of the Tooth Using an Innovative Plant Stain Found in the Pravara Region, India
Sanjay G Thete, Tejashree Mantri, Deepak Vikhe, Chaitra Mastud, Prasad Mhaske, Santoshkumar Pandurang Mastud
Abstract
AIMS: , commonly known as Harad, is found in South Asia from India. Few species are found in the Pravara region of Maharashtra, India. MATERIALS AND METHODS: A total of 40 extracted teeth were collected and ground sections of each tooth were prepared. The samples were randomly divided into two groups: group I-control (without stain), group II-study group (the samples in this group were stained with the help of innovative "Pravara" stain). The sections were graded from I to III according to the differentiation and contrast of tissues. RESULTS: The ground sections in group II stained with Pravara stain demonstrated better contrast for structures within sections under a microscope in comparison with group I (control group). CONCLUSION: The study demonstrates the efficacy of a new innovative Pravara stain, which is a simple, economical, quick, and effective staining method for the ground sections of the teeth. CLINICAL SIGNIFICANCE: This innovative Pravara stain is cost-effective, saves time, ecofriendly, and highlights the anatomical structures more effectively and does not fade easily with time.