Antioxidant, hypoglycemic and protection of acute liver injury activities of Ganoderma lucidum spore water extract
Long Zhang, Huiyu Qiao, Hongxing Liu, Guochuan Jiang, Liyan Wang, Xuejun Liu
Abstract
This study determined the chemical composition, antioxidant activity, hypoglycemic activity, and acute liver injury-protective effects of sporoderm-broken Ganoderma lucidum spore water extract (BGLWE). A total of 549 substances were identified in BGLWE, among which, the top three identified substances were nicotinamide (9.75 %), gamma-aminobutyric acid (9.44 %), and phytosphingosine (8.36 %). BGLWE exhibited pronounced scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and hydroxyl radicals (IC50 of 0.2456, 0.3637, and 3.573 mg/mL, respectively) and also possessed high ferric ion reducing power (absorbance from 0.22 to 3.06). BGLWE showed good inhibitory activities against α-amylase and α-glucosidase (IC50 of 4.87 and 5.06 mg/mL). In animal experiments, compared with the control CCl4 group, the enzymatic activities of glutathione peroxidase and superoxide dismutase of BGLWE were increased by 51.52 % and 41.79 %, respectively, while the malondialdehyde content was decreased by 53.99 %. These results support the use of BGLWE as a natural hypoglycemic and hepatoprotective agent that has potential applications in food and medicine.