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Recent Advances in Mass Spectrometry-Based Bottom-Up Proteomics

Cameron S. Movassaghi, Jie Sun, Yuming Jiang, Natalie Turner, Vincent Chang, Nara Chung, Ryan Chen, Elizabeth N. Browne, Chuwei Lin, Devin K. Schweppe, Stacy A. Malaker, Jesse G. Meyer

2025Analytical Chemistry45 citationsDOIOpen Access PDF

Abstract

Mass spectrometry-based proteomics is about 35 years old, and recent progress appears to be speeding up across all subfields. In this review, we focus on advances over the last two years in select areas within bottom-up proteomics, including approaches to high-throughput experiments, data analysis using machine learning, drug discovery, glycoproteomics, extracellular vesicle proteomics, and structural proteomics.

Topics & Concepts

ChemistryMass spectrometryProteomicsChromatographyBiochemistryGeneAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsBiosensors and Analytical Detection