A Shuttle-Vector System Allows Heterologous Gene Expression in the Thermophilic Methanogen Methanothermobacter thermautotrophicus ΔH
Christian Fink, Sebastian Beblawy, Andreas M. Enkerlin, Lucas Mühling, Largus T. Angenent, Bastian Molitor
Abstract
The world economies are facing permanently increasing energy demands. At the same time, carbon emissions from fossil sources need to be circumvented to minimize harmful effects from climate change.
Topics & Concepts
ThermophileFormate dehydrogenaseHeterologous expressionShuttle vectorPlasmidBioproductionMethanogenGeneBiologySelectable markerHeterologousEscherichia coliBiochemistryChemistryFormateHydrogenaseExpression vectorComputational biologyBacteriaDNATransformation (genetics)MethanogenesisCarbon fixationGene expressionMicrobiologyAnaerobic Digestion and Biogas ProductionMicrobial metabolism and enzyme functionMicrobial Fuel Cells and Bioremediation