Loosenin-Like Proteins from <i>Phanerochaete carnosa</i> Impact Both Cellulose and Chitin Fiber Networks
Mareike Monschein, Eleni Ioannou, Taru Koitto, Leamon A. K. M. Al Amin, Jutta J. Varis, Edward R. Wagner, Kirsi S. Mikkonen, Daniel J. Cosgrove, Emma R. Master
Abstract
The present study deepens investigations of microbial expansin-related proteins and their applied significance by (i) reporting a detailed comparison of diverse loosenins encoded by the same organism, (ii) considering both cellulosic and chitin-containing materials as targeted substrates, and (iii) investigating the impact of the C-terminal carbohydrate binding module (CBM) present in other expansin-related proteins on loosenin function. By revealing the potential of fungal loosenins to impact both cellulose and chitin-containing networks, our study reveals a possible biological and applied role of loosenins in fungal cell wall processing.