Extraction, Purification, Physicochemical Properties, and Activity of a New Polysaccharide From Cordyceps cicadae
Zizhong Tang, Wenjie Lin, Yusheng Chen, Shiling Feng, Yihan Qin, Yirong Xiao, Hong Chen, Hong Chen, Yuntao Liu, Hui Chen, Hui Chen, Tongliang Bu, Qinfeng Li, Yi Cai, Huipeng Yao, Chunbang Ding
Abstract
The polysaccharides from C. cicadae were extracted by ultrasonically-assisted enzymatic extraction (UAEE). Response surface analysis was used to determine the optimum parameters as follows: addition of enzymes, 0.71%; extraction temperature, 60°C; extraction time, 18 min; liquid-solid ratio, 46:1 (mL/g). The extraction yield of polysaccharide was 3.66 ± 0.87%. A novel polysaccharide fraction (JCH-a1) from C. cicadae was extracted and then purified by cellulose DEAE-32 and Sephadex G-100 anion exchange chromatography. The analysis results showed that the molar ratio of galactose, glucose, and mannose in JCH-a1 cells (60.7 kDa) was 0.89:1:0.39. JCH-a1 with a triple helix contains more α-glycosides and has strong thermal stability. Moreover, JCH-a1 showed strong antioxidant activity and acted as a strong inhibitor of α-glucosidase in vitro . In addition, JCH-a1 can prolong the lifespan of C. elegans . The present study might provide a basis for further study of JCH-a1 as an antioxidant and hypoglycemic food or drug.