Recent advances in the screening methods of NPC1L1 inhibitors
Renshuai Zhang, Wenjing Liu, Jun Zeng, Jingsen Meng, Lingyu Shi, Shanbo Yang, Jing Chang, Chao Wang, Kunyue Xing, Jialian Wen, Ning Liu, Bing Liang, Dongming Xing
Abstract
NPC1L1 is a crucial protein involved in sterol lipid absorption and has been shown to play an important role in intestinal cholesterol absorption. Hypercholesterolemia is a significant risk factor for cardiovascular diseases such as coronary heart disease. Screening of NPC1L1 inhibitors is critical for gaining a full understanding of lipid metabolism, developing new cholesterol-lowering medicines, and treating cardiovascular diseases. This work summarized existing methodologies for screening NPC1L1 inhibitors and evaluated their challenges, and will assist the development of novel cholesterol-lowering medications and therapeutic strategies for hypercholesterolemia and other cholesterol-related metabolic disorders.