Small Molecule‐Inducible and Photoactivatable Cellular RNA N1‐Methyladenosine Editing
Guoyou Xie, Yunqing Lu, Jiaxin He, Xianyuan Yang, Jiawang Zhou, Yi Cheng, Jian Li, Zigang Li, Gholamreza Asadikaram, Hongxin Niu, Xiao‐Feng Xiong, Jiexin Li, Hongsheng Wang
Abstract
Abstract N1‐methyladenosine (m 1 A) modification is one of the most prevalent epigenetic modifications on RNA. Given the vital role of m 1 A modification in RNA processing such as splicing, stability and translation, developing a precise and controllable m 1 A editing tool is pivotal for in‐depth investigating the biological functions of m 1 A. In this study, we developed an abscisic acid (ABA)‐inducible and reversible m 1 A demethylation tool (termed AI‐dm 1 A), which targets specific transcripts by combining the chemical proximity‐induction techniques with the CRISPR/dCas13b system and ALKBH3. We successfully employed AI‐dm 1 A to selectively demethylate the m 1 A modifications at A8422 of MALAT1 RNA, and this demethylation process could be reversed by removing ABA. Furthermore, we validated its demethylation function on various types of cellular RNAs including mRNA, rRNA and lncRNA. Additionally, we used AI‐dm 1 A to specifically demethylate m 1 A on ATP5D mRNA, which promoted ATP5D expression and enhanced the glycolysis activity of tumor cells. Conversely, by replacing the demethylase ALKBH3 with methyltransferase TRMT61A, we also developed a controllable m 1 A methylation tool, namely AI‐m 1 A. Finally, we caged ABA by 4,5‐dimethoxy‐2‐nitrobenzyl (DMNB) to achieve light‐inducible m 1 A methylation or demethylation on specific transcripts. Collectively, our m 1 A editing tool enables us to flexibly study how m 1 A modifications on specific transcript influence biological functions and phenotypes.