Protocol to perform dynamic microfluidic single-cell cultivation of C. glutamicum
Luisa Blöbaum, Sarah Täuber, Alexander Grünberger
Abstract
Here, we present a protocol for the design, fabrication, and usage of a polydimethylsiloxane (PDMS)-based chip for dynamic microfluidic single-cell cultivation of Corynebacterium glutamicum. We describe steps for flow profile establishment and biological preparation. We then detail time-lapse imaging to observe reactions of C. glutamicum to repeated environmental changes in the range of seconds. This system can be adapted to other organisms with a cell wall and soluble non-gaseous environmental factors like nutrients. For complete details on the use and execution of this protocol, please refer to Täuber et al..1
Topics & Concepts
Corynebacterium glutamicumPolydimethylsiloxaneMicrofluidicsProtocol (science)Computer scienceNanotechnologyChemistryMaterials scienceBiochemistryGenePathologyAlternative medicineMedicineMicrobial Metabolic Engineering and BioproductionViral Infectious Diseases and Gene Expression in Insects3D Printing in Biomedical Research