Enhancement of Sphingolipid Synthesis Improves Osmotic Tolerance of Saccharomyces cerevisiae
Guoxing Zhu, Nannan Yin, Qiuling Luo, Jia Liu, Xiulai Chen, Li Liu, Jianrong Wu
Abstract
This study demonstrated a novel strategy for the manipulation of membrane complex sphingolipids to enhance S. cerevisiae tolerance to osmotic stress. Elo2, a sphingolipid acyl chain elongase, was related to osmotic tolerance through transcriptome analysis of the wild-type strain and an osmosis-tolerant strain generated from ALE. Overexpression of ELO2 increased the content of complex sphingolipid with longer acyl chain; thus, membrane integrity and osmotic tolerance improved.
Topics & Concepts
Saccharomyces cerevisiaeOsmotic shockSphingolipidStrain (injury)Osmotic pressureMutantTranscriptomeWild typeBiologyBiochemistryYeastChemistryGeneGene expressionAnatomySphingolipid Metabolism and SignalingPhotosynthetic Processes and MechanismsLipid Membrane Structure and Behavior