Development and Application of a Nanobody-Based Competitive ELISA for Detecting Antibodies against Hepatitis E Virus from Humans and Domestic Animals
Yiyang Chen, Meimei Zhang, Tianxiang Chen, Jiaxi Wang, Qin Zhao, En‐Min Zhou, Baoyuan Liu
Abstract
HEV is thought to be a zoonotic infection and is widespread worldwide; it is beneficial to establish a more convenient and spectral method for HEV antibody detection. In this study, a convenient, time-saving, reproducible, highly sensitive, specific, and novel nanobody-based cELISA was developed and can be used to detect IgG antibodies against mammalian HEV. It provides a new technique for serological evaluation and ELISA-based diagnosis of HEV infection.
Topics & Concepts
VirologyAntibodyVirusHepatitis a virusBiologyImmunologyHepatitis Viruses Studies and EpidemiologyViral gastroenteritis research and epidemiologyVeterinary medicine and infectious diseases