Secretome Analysis Using Affinity Proteomics and Immunoassays: A Focus on Tumor Biology
Vanessa M. Beutgen, V. A. Shinkevich, Johanna Pörschke, Celina Meena, Anna Mary Steitz, Elke Pogge von Strandmann, Johannes Graumann, María Gómez‐Serrano
Abstract
The study of the cellular secretome using proteomic techniques continues to capture the attention of the research community across a broad range of topics in biomedical research. Due to their untargeted nature, independence from the model system used, historically superior depth of analysis, as well as comparative affordability, mass spectrometry-based approaches traditionally dominate such analyses. More recently, however, affinity-based proteomic assays have massively gained in analytical depth, which together with their high sensitivity, dynamic range coverage as well as high throughput capabilities render them exquisitely suited to secretome analysis. In this review, we revisit the analytical challenges implied by secretomics and provide an overview of affinity-based proteomic platforms currently available for such analyses, using the study of the tumor secretome as an example for basic and translational research.