Identification of the Immunoglobulin E Epitope of Arginine Kinase, an Important Allergen from <i>Crassostrea angulata</i>
Fei Huan, Shuai Gao, Tian-Jiao Han, Meng Liu, Mengsi Li, Yang Yang, Yiyu Chen, Dong Lai, Min‐Jie Cao, Guang‐Ming Liu
Abstract
was registered to the World Health Organization/International Union of Immunological Societies allergen nomenclature committee to be named as Cra a 2. The immunoglobulin G/immunoglobulin E-binding capacity of Cra a 2 was significantly reduced after chemical denaturation treatment. Further, eight linear mimotopes and five conformational mimotopes of Cra a 2 were obtained using phage panning. In addition to six linear epitopes that have been identified, two linear epitopes were verified by a synthetic peptide, of which L-Cra a 2-2 was conserved in shellfish. Four conformational epitopes were verified by site-directed mutation, among which mutation of C-Cra a 2-1 affected the structure and reduced the immunoreactivity of Cra a 2 most significantly. Overall, the identified epitopes may lay a foundation for the development of hypoallergenic oyster products through food processing.