Detection of Monkeypox Virus DNA in Airport Wastewater, Rome, Italy
Giuseppina La Rosa, Pamela Mancini, Carolina Veneri, Giusy Bonanno Ferraro, Luca Lucentini, M. Iaconelli, Elisabetta Suffredini
Abstract
Environmental surveillance can be a complementary tool for detecting pathogens circulating in communities. We detected monkeypox virus DNA in wastewater from Italy's largest airport by using real-time PCR assays targeting the G2R region and F3L and N3R genes and sequencing. Wastewater surveillance can be quickly adapted to investigate emerging threats.
Topics & Concepts
MonkeypoxVirologyWastewaterVirusDNA sequencingBiologyPolymerase chain reactionDNAGeneEnvironmental scienceVacciniaEnvironmental engineeringGeneticsRecombinant DNAPoxvirus research and outbreaksBacillus and Francisella bacterial researchBacteriophages and microbial interactions