Probing juxtaposed G-quadruplex and hairpin motifs using a responsive nucleoside probe: a unique scaffold for chemotherapy
Saddam Y. Khatik, Sruthi Sudhakar, Satyajit Mishra, Jeet Kalia, P. I. Pradeepkumar, Seergazhi G. Srivatsan
Abstract
F label enabled the assessment of ligand-induced changes in GQ dynamics. Studies also revealed that mutations in the hairpin domain affected GQ formation and stability, which was further functionally verified in polymerase stop assay. We anticipate that these findings and useful properties of the nucleoside probe could be utilized in designing and evaluating binders that jointly target both GQ and hairpin domains for enhanced selectivity and druggability.
Topics & Concepts
ChemistrySmall moleculeBiophysicsContext (archaeology)PopulationComputational biologyStereochemistryBiologyBiochemistryDemographySociologyPaleontologyDNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery