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Total Synthesis of (−)‐Batrachotoxin Enabled by a Pd/Ag‐Promoted Suzuki–Miyaura Coupling Reaction

Yuuki Watanabe, Hisahiro Morozumi, Hiroyuki Mutoh, Koichi Hagiwara, Masayuki Inoue

2023Angewandte Chemie International Edition18 citationsDOIOpen Access PDF

Abstract

Abstract Batrachotoxin is an extremely potent cardio‐ and neurotoxic steroidal alkaloid found in certain species of frogs, birds, and beetles. The steroidal 6/6/6/5‐membered carbocycle (ABCD‐ring) is U‐shaped and functionalized with two double bonds, a six‐membered C3‐hemiacetal across the AB‐ring, a seven‐membered oxazepane on the CD‐ring, and a dimethylpyrrolecarboxy group at the D‐ring carbon chain. These structural features present an unusual and formidable synthetic challenge. Herein we report a total synthesis of batrachotoxin based on a newly devised convergent strategy through a 22‐step sequence. Enantiopure AB‐ring and D‐ring fragments were prepared and subjected to a crucial C(sp 2 )−C(sp 2 ) coupling reaction. Although both C(sp 2 ) centers were sterically encumbered by proximal tetrasubstituted carbon atoms, Ag 2 O strongly promoted the Pd(PPh 3 ) 4 ‐catalyzed Suzuki–Miyaura coupling reaction at room temperature, thereby connecting the two fragments without damaging their preexisting functionalities. Subsequent treatment with t ‐BuOK induced Dieckmann condensation to cyclize the C‐ring. The judiciously optimized functionalizations realized oxazepane formation, carbon chain extension, and pyrrole carboxylic acid condensation to deliver batrachotoxin.

Topics & Concepts

BatrachotoxinChemistryRing (chemistry)StereochemistrySuzuki reactionEnantiopure drugTotal synthesisCoupling reactionCondensation reactionEnantioselective synthesisOrganic chemistryCatalysisPalladiumSodium channelSodiumMarine Sponges and Natural ProductsAlkaloids: synthesis and pharmacologyChemical synthesis and alkaloids