Adaptation of the gut pathobiont Enterococcus faecalis to deoxycholate and taurocholate bile acids
Francis Repoila, F. Le Bohec, Cyprien Guérin, Caroline Lacoux, Sandeep Tiwari, Arun Kumar Jaiswal, Mariana Passos Santana, Sean Kennedy, Benoît Quinquis, Dominique Rainteau, Vincent Juillard, Sylviane Furlan, Philippe Bouloc, Pierre Nicolas, Anderson Miyoshi, Vasco Azevedo, Pascale Serror
Abstract
Enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. This bacterial species is subdominant in a healthy physiological state of the gut microbiota (eubiosis) in adults, but can become dominant and cause infections when the intestinal homeostasis is disrupted (dysbiosis). The relatively high concentrations of bile acids deoxycholate (DCA) and taurocholate (TCA) hallmark eubiosis and dysbiosis, respectively. This study aimed to better understand how E. faecalis adapts to DCA and TCA. We showed that DCA impairs E. faecalis growth and possibly imposes a continuous adjustment in the expression of many essential genes, including a majority of ribosomal proteins. This may account for slow growth and low levels of E. faecalis in the gut. In contrast, TCA had no detectable growth effect. The evolving transcriptome upon TCA adaptation showed the early activation of an oligopeptide permease system (opp2) followed by the adjustment of amino acid and nucleotide metabolisms. We provide evidence that TCA favors the exploitation of oligopeptide resources to fuel amino acid needs in limiting oligopeptide conditions. Altogether, our data suggest that the combined effects of decreased DCA and increased TCA concentrations can contribute to the rise of E. faecalis population during dysbiosis.