Fluorescent annulated imidazo[4,5-<i>c</i>]isoquinolines<i>via</i>a GBB-3CR/imidoylation sequence – DNA-interactions in pUC-19 gel electrophoresis mobility shift assay
Mareen Stahlberger, O. Steinlein, C. R. Adam, M. Rotter, Jens Hohmann, Martin Nieger, Beate Köberle, Stefan Bräse
Abstract
]isoquinolines comprising a GBB-3CR, followed by an intramolecular imidoylative cyclisation. X-Ray crystallography revealed a flat 3D structure of the obtained polyheterocycles. Thus, we evaluated their interactions with double-stranded DNA by establishing a pUC-19 plasmid-based gel electrophoresis mobility shift assay, revealing a stabilising effect on ds-DNA against strand-break inducing conditions.
Topics & Concepts
ChemistryFluorescenceElectrophoretic mobility shift assayDNASequence (biology)Gel electrophoresisStereochemistryBiochemistryGenePhysicsGene expressionQuantum mechanicsChemical Synthesis and AnalysisClick Chemistry and ApplicationsSynthesis and Biological Evaluation