Proteomic Changes of Klebsiella pneumoniae in Response to Colistin Treatment and <i>crrB</i> Mutation-Mediated Colistin Resistance
Lang Sun, Pernille Kronholm Rasmussen, Yinlei Bai, Xiulan Chen, Tanxi Cai, Jifeng Wang, Xiaojing Guo, Zhensheng Xie, Xiang Ding, Lili Niu, Nali Zhu, Xuefu You, Finn Kirpekar, Fuquan Yang
Abstract
Polymyxins are increasingly used as the critical last-resort therapeutic options for multidrug-resistant Gram-negative bacteria. Unfortunately, polymyxin resistance has increased gradually over the past few years. Although studies on polymyxin mechanisms are expanding, systemwide analyses of the underlying mechanism for polymyxin resistance and stress response are still lacking. To understand how Klebsiella pneumoniae adapts to colistin (polymyxin E) pressure, we carried out proteomic analysis of a K. pneumoniae strain cultured with different concentrations of colistin.