Geometric Remodeling of Nitrilase Active Pocket Based on ALF-Scanning Strategy To Enhance Aromatic Nitrile Substrate Preference and Catalytic Efficiency
Zi-Kai Wang, Jin‐Song Gong, Dan-Ting Feng, Chang Su, Hui Li, Zhiming Rao, Zhen‐Ming Lu, Jin‐Song Shi, Zhenghong Xu
Abstract
In this study, the active pocket was largely remodeled based on the ALF-scanning strategy we developed. It is believed that ALF-scanning not only could be employed for substrate preference modification but might also play a role in protein engineering of other enzymatic properties, such as substrate region selectivity and substrate spectrum. In addition, the mechanism of aromatic nitrile substrate adaptation we found is widely applicable to other nitrilases in nature. To a large extent, it could provide a theoretical basis for the rational design of other industrial enzymes.
Topics & Concepts
Substrate (aquarium)NitrileNitrilaseSelectivityProtein engineeringCatalysisChemistryActive siteCombinatorial chemistryMaterials scienceStereochemistryEnzymeBiochemistryOrganic chemistryBiologyEcologyPeptidase Inhibition and AnalysisPancreatic function and diabetesBiochemical and Molecular Research