Enzymatic blood group conversion of human kidneys during <i>ex vivo</i> normothermic machine perfusion
Serena MacMillan, Sarah A. Hosgood, Michael L. Nicholson
Abstract
A major restriction to transplantation is the requirement for ABO blood group compatibility between donor and recipient. In this study, an α-galactosidase enzyme from Bacteroides fragilis was used successfully to remove type B blood group antigens enzymatically from human kidneys using ex vivo normothermic machine perfusion. This provides the first step for a strategy to overcome the ABO barrier in kidney transplantation.
Topics & Concepts
ABO blood group systemMedicineEx vivoBacteroides fragilisMachine perfusionPerfusionTransplantationKidneyIn vivoKidney transplantationBlood type (non-human)UrologyImmunologySurgeryInternal medicineBiochemistryBiologyLiver transplantationAntibioticsBiotechnologyRenal Transplantation Outcomes and TreatmentsOrgan Transplantation Techniques and OutcomesOrgan Donation and Transplantation