Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay
Lennart J. K. Weiß, Philipp Rinklin, Bhawana Thakur, Emir Music, Heike Url, Inola Kopic, Darius Hoven, M. Banzet, Tassilo von Trotha, Dirk Mayer, Bernhard Wolfrum
Abstract
This work demonstrates a lateral flow assay concept on the basis of stochastic-impact electrochemistry. To this end, we first elucidate requirements to employ silver nanoparticles as redox-active labels. Then, we present a prototype that utilizes nanoimpacts from biotinylated silver nanoparticles as readouts to detect free biotin in solution based on competitive binding. The detection is performed in a membrane-based microfluidic system, where free biotin and biotinylated particles compete for streptavidin immobilized on embedded latex beads. Excess nanoparticles are then registered downstream at an array of detection electrodes. In this way, we establish a proof of concept that serves as a blueprint for future "digital" lateral flow sensors.