PEDV-spike-protein-expressing mRNA vaccine protects piglets against PEDV challenge
Yongxiang Zhao, Baochao Fan, Xu Song, Jie Gao, Rongli Guo, Yi Cheng, Zhaoming He, Hongpeng Hu, Jianhao Jiang, Lixiang Zhao, Tianyi Zhong, Bin Li
Abstract
. Importantly, the S mRNA-LNP vaccine not only protected actively immunized piglets against PEDV but also equipped neonatal piglets with effective passive anti-PEDV immunity in the form of colostrum-derived antibodies after the immunization of sows. Our findings suggest that the PEDV-S mRNA-LNP vaccine is a promising candidate for combating PEDV infection.IMPORTANCEPorcine epidemic diarrhea virus (PEDV) continues to harm the global swine industry. It is important to develop a highly effective vaccine to control PEDV infection. Here, we report a PEDV spike (S) mRNA vaccine that primes a potent antibody response and antigen-specific T-cell responses in immunized piglets. Active and passive immunization can protect piglets against PED following the virus challenge. This study highlights the efficiency of the PEDV-S mRNA vaccine and represents a viable approach for developing an efficient PEDV vaccine.