Artificial Peroxisome <i>h</i>NiPt@Co-NC with Tetra-enzyme Activities for Colorimetric Glutathione Sensing
Tingting Zhang, Nannan Lu, Chao Wang, Hongfei Jiang, Miao Zhang, Renshuai Zhang, Yingjie Zhong, Dongming Xing
Abstract
Artificial peroxisome plays an important part in protocell system construction and disease therapy. However, it remains an enormous challenge to exploit a practicable artificial peroxisome with multiple and stable activities. Nanozymes with multienzyme mimetic activities stand out for artificial peroxisome preparation. Herein, a novel nanozyme─Co-nanoparticle-embedded N-enriched carbon nanocubes (Co,N-CNC) decorated by hollow NiPt nanospheres ( h NiPt@Co-NC) with featured tetra-enzyme mimetic activities of natural peroxisome─was prepared. Due to the synergistic effect of hollow NiPt nanospheres ( h NiPtNS) and cubic porous Co,N-CNC support, h NiPt@Co-NC exhibited oxidase (OXD), peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD)-like activities with comparable catalytic efficiency, enabling it to be a competitive candidate for artificial peroxisome investigation. Based on the high OXD-mimetic activity of h NiPt@Co-NC, a facile colorimetric platform was proposed for reduced glutathione (GSH) detection with a wide linear range (0.1–5 μM, 5–100 μM) and a low detection limit (27 nM). Thus, the h NiPt@Co-NC with tetra-enzyme mimetic activities possessed bright prospects in diversified biotechnological applications, including artificial organelles, biosensing, and medical diagnostics.