Asymmetric Si-rhodamine scaffolds: rational design of pH-durable protease-activated NIR probes <i>in vivo</i>
Min Li, Chuanfeng Wang, Ting Wang, Mengting Fan, Ning Wang, Danying Ma, Ting Hu, Xiaoyan Cui
Abstract
A group of asymmetric Si-rhodamine scaffolds was designed for protease-activated NIR probes. Dual pH-inertia for both spirocyclized fluorescent probes and fluorescent products of zwitterions form over a wide range of pH (4.0-11.0). Leucine aminopeptidase (LAP) and γ-glutamyl transpeptidase (GGT) were monitored by fluorescent imaging in vivo.
Topics & Concepts
In vivoRational designChemistryRhodamineRhodamine BCombinatorial chemistryNanotechnologyMaterials scienceBiophysicsFluorescenceOrganic chemistryCatalysisPhotocatalysisBiotechnologyPhysicsQuantum mechanicsBiologyAdvanced biosensing and bioanalysis techniquesClick Chemistry and ApplicationsRNA Interference and Gene Delivery