Kinetic modeling of Chinese hamster ovary cell culture: factors and principles
Peifeng Tang, Jianlin Xu, Alastair Louey, Zhijun Tan, Andrew Yongky, Shaoyan Liang, Zheng Jian Li, Yongyan Weng, Shijie Liu
Abstract
As a host for therapeutic protein expression, Chinese hamster ovary (CHO) cells are widely utilized in the mainstream biopharmaceutical industry. Cell culture process development plays an important role in transitioning laboratory research to manufacturing. Among different mathematic tools, kinetic modeling is commonly achieved through analyzing cell culture data to design process parameters, optimize media, and scale up bioreactors. In this review, we examine key factors for upstream process development, and summarize currently used kinetic modeling strategies. In addition, two original examples of kinetic modeling application optimizing cell culture performance are presented. A comprehensive understanding is provided for the kinetic modeling and its applications in cell culture process development.