Undervalued Pseudo- <i>nifH</i> Sequences in Public Databases Distort Metagenomic Insights into Biological Nitrogen Fixers
Kazumori Mise, Yoko Masuda, Keishi Senoo, Hideomi Itoh
Abstract
Nitrogen-fixing microbes affect biogeochemical cycling, agricultural productivity, and microbial ecosystems, and their distributions have been investigated intensively using genomic and metagenomic sequencing. Currently, insights into nitrogen fixers in the environment have been acquired by homology searches against nitrogenase genes, particularly the nifH gene, in public databases.
Topics & Concepts
MetagenomicsNitrogen fixationBiogeochemical cycleBiologyNitrogenaseNitrogen cycleGeneBiodiversityEcosystemComputational biologyEvolutionary biologyEcologyGeneticsNitrogenChemistryBacteriaOrganic chemistryWastewater Treatment and Nitrogen RemovalMicrobial Community Ecology and PhysiologyAmmonia Synthesis and Nitrogen Reduction