Litcius/Paper detail

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing

Joseph M. Replogle, Thomas M. Norman, Albert Xu, Jeffrey A. Hussmann, Jin Chen, J. Zachery Cogan, Elliott J. Meer, Jessica M. Terry, Daniel P. Riordan, Niranjan Srinivas, Ian T. Fiddes, Joseph G. Arthur, Luigi J. Alvarado, Katherine A. Pfeiffer, Tarjei S. Mikkelsen, Jonathan S. Weissman, Britt Adamson

2020Nature Biotechnology494 citationsDOIOpen Access PDF

Topics & Concepts

CRISPRCRISPR interferenceSubgenomic mRNAGuide RNAComputational biologyBiologyCas9Trans-activating crRNASingle-cell analysisGeneticsGenome editingBiogenesisRNAGeneCellCRISPR and Genetic EngineeringSingle-cell and spatial transcriptomicsRNA and protein synthesis mechanisms