Efficient bioconversion of high-concentration <scp>d</scp>-fructose into <scp>d</scp>-mannose by a novel <i>N</i>-acyl-<scp>d</scp>-glucosamine 2-epimerase from <i>Thermobifida halotolerans</i>
Peng Jin, Yuanyuan Wang, Zhengang Liang, Yuan Miao, Hua Li, Qizhen Du
Abstract
A novel <italic>N</italic>-acyl-<sc>d</sc>-glucosamine 2-epimerase ThMI exhibits high mannose isomerase activity with a maximum bioconversion ratio of 35.8% in 500 g L<sup>−1</sup><sc>d</sc>-fructose. Whole-cell biocatalyst produced 157 g L<sup>−1</sup><sc>d</sc>-mannose from 500 g L<sup>−1</sup><sc>d</sc>-fructose in 60 min.
Topics & Concepts
BioconversionChemistryFructoseMannoseGlucose-6-phosphate isomeraseStereochemistryBiochemistryEnzymeFermentationDiet, Metabolism, and DiseasePolyamine Metabolism and ApplicationsCarbohydrate Chemistry and Synthesis