α-Pyrone Derivatives from <i>Calcarisporium arbuscula</i> Discovered by Genome Mining

Abstract

A highly reducing polyketide synthase (HRPKS) gene cluster from the genome of Calcarisporium arbuscula was identified through genome mining. Heterologous expression of this cluster led to the production of four new α-pyrone compounds, calcapyrones A ( 1 ) and B ( 2 ), along with their biosynthetic intermediates calcapyrones C ( 3 ) and D ( 4 ). The structures of these compounds were elucidated on the basis of extensive spectroscopic experiments, and the absolute configurations of the 7,8-diol moieties in 1 and 2 were assigned using Snatzke’s method. The biosynthetic pathway of 1 and 2 was established through in vivo and in vitro experiments.

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