Photocatalytic Dye Degradation and Bacteriostatic Efficacy of Myco Synthesized Copper Nanoparticles by new Isolate Lentinus squarrosulus (Mont.) from Dead Trunk of Nerium odourum

Abstract

Objectives: To investigate the antibacterial activity and photo-catalytic efficacy of CuNPs synthesized from Lentinus squarrosulus used for the degradation of commercial textile dyes. Methods: The Copper nanoparticles (CuNPs) were biosynthesized using L. squarrosulus, a new fungal isolate from the dead trunk of N. odorum. The production of copper nanoparticles using mycoextract was simple and environmentally friendly, yielding stable copper nanoparticles in a variety of shapes. Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), and EDAX analysis have all been used to establish that CuNPs were successfully formed. Findings: The research findings of the present study show that absorption spectra around 300 to 350 nm in UV-vis spectra followed by the presence of FT-IR peaks at 3680cm􀀀1 and 3856.7cm􀀀1 of proteins, carbohydrates, flavonoids, and tannins confirmed the myco-synthesis of copper nanoparticles using L. squarrosulus. Additionally, scanning electron microscopy examination of their external morphology reveals the existence of CuNPs with an average particle size of about 200 nm each, which are the primary spherical nanoparticles. Energy-dispersive x-ray (EDX) analysis was performed to further investigate the configuration of copper nanoparticles and it was found that, pure copper (03.51 percent) was present in CuNPs. The produced CuNPs were assessed for their antimicrobial activity and the results show that they were highly effective against Enterobacter aerogenes MTCC 2823 and Streptococcus mutans MTCC 497, while Escherichia coli MTCC 739 and Enterococcus faecalis MTCC 439 showed moderate antibacterial activity. Further, the photocatalytic dye degradation potentials of myco-synthesized CuNPs showed higher degradation efficiency in fast green dye, moderate degradation rate in Congo red dye and a very low degradation rate in the brilliant blue dye. Novelty : Preparation of mycogenic copper nanoparticlesfrom L. squarrosulus and their photocatalytic degradation of organic dyes are the novel part in this study as there is no remarkable study in specific CuNPs. Keywords: Lentinus Squarrosulus; Antibacterial; Green Synthesis; Photocatalytic; Dye Degradation; Copper Nanoparticles

Topics & Concepts