High-Efficiency Protection of Linear DNA in Cell-Free Extracts from <i>Escherichia coli</i> and <i>Vibrio natriegens</i>
Masoud Norouzi, Sabina Panfilov, Keith Pardee
Abstract
extracts, and demonstrate that this simple and easily implemented method can enable an unprecedented plasmid-level expression from linear templates in this emerging chassis organism.
Topics & Concepts
Synthetic biologyBiomanufacturingEscherichia coliComputational biologyBiologyPlasmidmCherryDNATemplateCell-free protein synthesisGeneGeneticsComputer scienceGreen fluorescent proteinProtein biosynthesisProgramming languageCRISPR and Genetic EngineeringBacterial Genetics and BiotechnologyRNA Interference and Gene Delivery