Molecular Survey and Genetic Diversity of Babesia spp. and Theileria spp. in Cattle in Gansu Province, China
Ming Sun, Guiquan Guan, Zhijie Liu, Jinming Wang, Duoquan Wang, Shufang Wang, Chao Ma, Shengzhong Cheng, Hong Yin, Jianxun Luo
Abstract
PURPOSE: Babesia spp. and Theileria spp. are tick-borne pathogens of livestock globally. In this study, we investigated the presence and distribution of these pathogens in cattle from 20 locations in 4 Counties of Wuwei City. The aim of the present research was to evaluate the spread of piroplasms, so as to provide the epidemiological information for control piroplasmosis in the region. METHODS: The authors provided the molecular data for Babesia spp. and Theileria spp. and analyzed the obtained sequences of the 18S rRNA gene, Tams1 gene and MPSP gene by using the ClustalW program in MEGA version 6.06 software and BLASTn tool of NCBI GenBank database. RESULTS: The total infection rates were detected by nPCR with 1.8% for T. orientalis, 3% for T. sinensis, 0.6% for T. annulata, 1.8% for B. motasi and 0.6% for B. bigemina. CONCLUSIONS: To the best of our knowledge, this is the first report investigating T. sinensis from cattle by PCR in Wuwei City. In particular, ovine B. motasi has been for the first time detected in cattle in our study and its impact is worth discussing to figure out the potential reasons.